The effect of pH on three different enzymes Which of the following actions will provide the most appropriate negative control for the.

The results of the experiment are represented in Figure 1. The effect of pH on peroxidase activityOne of the researchers proposes using oxygen gas production to measure reaction rates. To test the claim, the researcher carries out an experiment that includes three different enzymes: pepsin, salivary amylase, and arginase. This is done to control for the placebo effect and to provide a baseline set of measurements for comparison to the primary experiment. The results of the experiment are represented in Figure 1. A negative control is an experiment that is run in parallel to a primary experiment with the same procedures except that the treatment is changed to something that is predicted to have no result. Finally, the researchers added the same amount of peroxidase to each test tube and measured the rate of each reaction at 23☌. The researchers included the guaiacol because it caused the solutions to change color as the reactions proceeded, which the researchers relied on for measuring reaction rates.

In an experiment, the researchers added a hydrogen peroxide solution containing guaiacol to several identical test tubes and adjusted the solution in each test tube to a different pH. Negative Controls are characterized by the absence of reagents or components that are necessary for successful analyte detection. Researchers investigated the influence of environmental pH on the activity of peroxidase, an enzyme that catalyzes the conversion of hydrogen peroxide to water and oxygen gas.

Negative control for enzyme experiment